Journal: Chemical Science

Article Title: Unveiling the molecular dynamics of a nitrile-containing 5-lipoxygenase-activating protein antagonist in primary macrophages through Raman spectroscopy

doi: 10.1039/d5sc09493c

Figure Lengend Snippet: Subcellular colocalization analysis of 17-octadecynoic acid (17-ODYA) and BRP-685. (A) Representative SRS images of M1 macrophages showing the C C stretching mode of 17-ODYA (red, left), the C N stretching mode of BRP-685 (blue, middle), and the CH 3 stretching mode (green, right). To eliminate non-specific background, the off-resonant signal at 2500 cm −1 was subtracted from each of the displayed images. Three treatment conditions are displayed: 17-ODYA only (top), 17-ODYA + BRP-685 (middle), and BRP-685 only (bottom). The far-right column shows representative SRS spectra of the silent wavenumber region from lipid droplet areas. (B) Subcellular colocalization analysis of the C C and C N bands based on spontaneous Raman spectra, as described in the Methods section (SI). The area under the respective Raman bands was normalized by the DNA signal in the nucleus. In the back projection image, pixels were colored according to the corresponding segments in the scatter plot. Pixels with negative values in either the x - or y -coordinate of the scatter plot are shown in black. The nucleus is indicated by a semi-transparent white contour overlaid on the back-projection image.

Article Snippet: SRS images were recorded with a commercial multiphoton microscope optimized for nonlinear coherent Raman scattering (Leica Stellaris 8 CRS, Leica Microsystems GmbH, Wetzlar, Germany) as earlier described.

Techniques:

Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Fluorescent nanodiamonds engage innate immune effector cells: a potential vehicle for targeted anti-tumor immunotherapy

doi: 10.1016/j.nano.2016.12.005

Figure Lengend Snippet: (A) Visualization of micron-sized diamonds and evaluation of nitrogen vacancy center fluorescence emission was performed using an Olympus FV1000-Filter Confocal System, employing a 543 nm laser for excitation. Maximal emission was detected between 655–755 nm. (B) FNDs were examined on a Horiba ARAMIS Raman microscope using a 50× objective and excited with a 532 nm laser to determine the fluorescence emission spectrum. (C) FND morphology was evaluated using a high resolution scanning electron microscope (SEM). (D) FND size (in nm) was characterized by SEM, and a frequency histogram of the particle size distribution was generated.

Article Snippet: The fluorescence emission spectrum was obtained using a Horiba ARAMIS Raman upright microscope (50× objective) and a 532 nm excitation laser.

Techniques: Fluorescence, Microscopy, Generated

Journal: Materials

Article Title: Effects of Corrosion Products Deposited on 304 Stainless Steel on Reduction of Se (IV/VI) in Simulated Groundwater

doi: 10.3390/ma15082705

Figure Lengend Snippet: The Raman spectra of 304 stainless-steel samples after a 4 week immersion test in different solutions: (a) SG+ Na 2 SeO 3 ; (b) SG+ Na 2 SeO 4 .

Article Snippet: The formation mechanism of corrosion products deposited on the surface of 304 stainless-steel samples after the 4 week immersion test was analyzed by a confocal Raman microscope (CRM, Renishaw, London, UK).

Techniques: